cardiac puncture blood collection mouse

10-14 total fecal pellets and fur swabs may be pooled for each room. The most common approach is to lay the mouse on its back and insert a 25 to 30 gauge needle attached to a 1ml syringe just behind the xiphoid cartilage and slightly left of the middle. Lab Animal; 29(10):47-53. • The approximate blood volume of a mouse is 77-80 µl/g. Maximum blood volumes should be taken only from healthy animals (1). The samples were collected by cardiac puncture. These differences may result from peripheral capillary stasis and resistance [9] , stress response induced by collection technique [4] , or immunological demand of the host at various sites. Mouse and Rat Leukocytes Immunofluorescent Staining of Mouse and Rat Leukocytes I. Found inside – Page 33427.2.4.1 Blood Collection Mouse Blood 1. To perform cardiac puncture, euthanize mice using carbon dioxide. Then access the ventricle with an 18 gage needle and aspirate 400–500 μL blood into a 2 mL syringe. Terminal cardiac blood collection. For survival blood collection, the frequency and quantity of sample collection is dependent on the circulating blood volume and the rate of red blood cell turnover of the animal and varies by species. Abstract. If 15-20% of total blood volume is removed, cardiac output and blood pressure will be reduced. Blood collection requiring anesthesia (local/general anesthesia) Tail vein (rat, mice) Tail snip (mice) Orbital sinus (rat, mice) Jugular vein (rat, mice) Temporary cannula (rat, mice) Blood vessel cannulation (rat, guinea pig, ferret) Tarsal vein (guinea pig) Marginal ear vein/artery (rabbit) Terminal procedure Cardiac puncture (rat, mice, guinea pig, rabbit, ferret) Rodent Procedures . It is appropriate for all strains of mouse. Cardiac puncture (Terminal Procedure) However, they did not comprehensively evaluate collection sites nor utilize current anesthetics, … Considerations a. Found inside – Page 606Tail vein venipuncture in mice: A scalpel blade and a 25–30-gauge needle are required. The mouse is restrained ... Cardiac puncture represents an accepted method of blood collection from mice when more than a few drops are required. 0000000016 00000 n Pig venipuncture of the marginal ear vein v. Canine and feline venipuncture of the cephalic vein f. Cardiac puncture blood collection in rodents g. Cervical dislocation in mice . Found inside – Page 1325Blood collection from mice is complicated by size and volume limitations , and terminal procedures are often used ( e.g. , cardiac puncture or sampling of the posterior vena cava or abdominal aorta at necropsy ) ... 10-14 total fecal pellets and fur swabs may be pooled for each room. d. Fur swabs of each sentinel mouse and fresh fecal sample from each mouse in each sentinel cage in the room are pooled. Options include capillary, split-septum, push-caps and screw on, with a wide variety of volumes and additives. The SWMS Mouse – Blood Collection contains the following sections: o Legislation ... o NHMRC Blood Collection Guidelines o Saphenous Vein Puncture o Tail Vein Puncture Method o Facial Vein (Cheek Bleed) o Cardiac Puncture - Terminal . Cardiac puncture; a standard effective method requires anesthesia and is a terminal procedure while facial vein technique allows multiple collections. This well-illustrated edition offers new and updated chapters including immunology, viruses and parasites, behavior, enrichment and care standards of laboratory mice across the life sciences, medical and veterinary fields. Rabbit venipuncture of the marginal ear vein iv. The method is appropriate for all strains, this is a blood collection technique to obtain a single, large, good quality blood sample from a euthanized mouse or a mouse under terminal anaesthesia. 4. A report of the Nuffield Council on Bioethics working party investigating the ethical issues of research involving animals. cardiac puncture in micemethod for blood collectionponction cardiaque souris Use a 23g needle to puncture the vein and then immediately turn the rat back into a prone position and allow the blood to drip into a collection tube. Blood withdrawal by cardiac puncture or axillary cut down are considered terminal procedures and must be performed only after ensuring that the animal is under surgical anesthesia. bòÏ͖(ìB€{³Œj³€G¤Ì§èoÀ;Á!Ðü'ìäGÃe7ZšaÛïKÀ“Z™ÅÖRî:ûú7KÿjIA"©¢E(/é’íÁæw˞¿˜ 0À4€1fÕª#‘%ûPA5 ?óh…PWšBZ£ByeeõÆa3eá`‚’– Ȩô‘ÂVªRBC…f§ˆD^Q†Âaûöuòr4Q¯½úÑ"4e¡\+*EcsvÐ0ž-:Í›¸*:MªY{~»b.ßàCÉDéS$‘“ÃØpTLöKÎ. 1. 0000001622 00000 n The mice were under light anesthesia during the procedure. 0000008700 00000 n Cardiac puncture Anaesthesia or cervical dislocation required for all exsanguination sites/methods. 0000000993 00000 n Classic studies have reported differences in CBC and chemistry values from limited sampling sites in rodents 2;4;7–13. Cardiac Puncture Cardiac puncture was performed following the saphenous vein procedure while the mouse was under anesthesia with inhaled methoxyflurane. SOP: Blood Collection in the Mouse, Retro-orbital Bleed These SOPs were developed by the Office of the University Veterinarian and reviewed by Virginia Tech IACUC to provide a reference and guidance to investigators during protocol preparation and IACUC reviewers during protocol review. 0000008208 00000 n Anesthesia of laboratory animals • Animals undergoing potentially painful procedures should be provided with adequate anesthesia –Inhalant anesthetics –Injectable anesthetics –Immersion anesthetics –Local anesthetics 34 35. I would like to isolate PBMCs from mouse blood (cardiac puncture). Training 5) Blood collection a. Insert 1mL syringe with needle into the right ventricle and slowly draw blood, taking care not to draw so quickly that the heart immediately collapses. Blood Collection (Note: Navigation around this large pdf document is best accomplished using the bookmarks function.) Blood was serially diluted and cultured on a tryptic soy blood agar plate at 37 °C for 48 h (n = 10 mice). Researchers collect serial blood samples from several sites in the mouse, including the tail vein, saphenous vein, brachial vessels and the vena cava 1.As cardiac puncture is considered a … which of the following statements concerning health risks from working with mice is false . 4.4. Technique. Blood sampling in laboratory mice is a routine experimental procedure. Hemostasis: After a survival blood collection procedure, an alien cannot be returnedto its cage lock it has achieved complete hemostasis. : Blood collection is critical for mouse research studies particularly in hemostatic testing. The best results are obtained when administered 1015 minutes prior to blood - collection. The Laboratory Rat, Second Edition features updated information on a variety of topics including: rat genetics and genomics, both spontaneous and induced disease; state-of-the-art technology for housing and husbandry; occupational health, ... Fig. 0000000916 00000 n They can be used as referenced descriptions for procedures on IACUC protocols. The mouse is an increasingly important system for the study of both normal and aberrant hematopoiesis. Common sites for blood collection in mice include the retro-orbital sinus, facial vein (superficial temporal or submandibular veins), tail vein, saphenous vein, and heart (cardiac puncture) 2;4–6. Animal training to facilitate blood collection may reduce stress for certain species during the procedure. Pig venipuncture of the marginal ear vein v. anine and feline venipuncture of the cephalic vein f. ardiac puncture blood collection in rodents g. ervical dislocation in mice h. Neonatal rodent decapitation PART II 1. This book rectifies the problem by providing animal researchers and technicians with the essentials for conducting their work in the laboratory, offering detailed protocols and information that can be referred to on a daily basis. Collection from the auricular vein or artery of the rabbit is also relatively unstressful to the animal. This venous sinus is located just underneath the conjunctival membrane. true. In many protocols, multiple blood draws are an important part of collecting and analyzing data. Cardiac puncture was quoted as the preferred site for the guineapig, but ‘anaesthesia/analgesia should be given’. Nerenberg ST, Zedler P, Prasad R, Biskup N, Pedersen L. 1978. Obtainable volume: medium to large. c. Cut pericardial fat so that the heart is free and does not stick to the chest wall. xref The improved method involved the use of a restraining board instead of manual holding. Animal experimentation has been one of the most controversial areas of animal use, mainly due to the intentional harms inflicted upon animals for the sake of hoped-for benefits in humans. Jetzt auch in englischer Sprache! Technique Example: Central Artery Place the rabbit in a rabbit restrainer. Any post procedural outcome resulting in evident pain, discomfort or distress such as that associated with decreased appetite/ activity level, adverse reactions, to touch, open skin lesions, abscesses, lameness, conjunctivitis, corneal edema and photophobia. Issues that should guide the choice of survival blood collection route(s) are listed below, and an abbreviated summary is provided as Table 3. Methods of blood collection in the mouse. 355.1 Preface Blood collection (venipuncture, phlebotomy) is a common and important specimen collection procedure in the conduct of research. C1ƒ2ërቒ³10ÌX•ÄËpG`÷‹‚Ã\þ0-€¨gd`0¹¤™€Ø Blood Collection From Cardiac Puncture in Mouse Anesthetize a mouse by intra- peritoneal injection of Hypnorm Disinfect the thorax area with 75% alcohol cotton ball Insert a 24G 1” needle through the thoracic wall at the point of maximum heart palpitation Search for the maximum heart palpitation with your finger Withdraw blood slowly by your right hand Make an incision on the ventral aspect of the neck, just over the salivary glands. • The Circulating Blood Volume (CBV) of an adult rat is ~64 ml/kg (0.064ml/g). 355.3.7.4 Serial micro-sampling in rodents, such as blood glucose monitoring for example, can be accomplished by using a combination collection technique consisting of a single, initial tail vein nick (50uL blood drop) Cardiac puncture; a standard effective method requires anesthesia and is a terminal procedure while facial vein technique allows multiple collections. Blood Collection Recommended Sites jugular, saphenous, pedal vein, tail vein/artery, cardiac puncture Injection Sites and Volumes *Use the smallest size needle gauge possible when performing injections. ˆYœ÷@ÄÙ. Rat and mouse venipuncture of the lateral tail vein iii. Cardiac puncture is done only as a terminal procedure and always under anesthesia as evidenced by lack of response to a painful stimulus (e.g., toe pinch) The use of a 1cc syringe with a 25 gauge needle is recommended. Lab Anim. A.Cardiac Puncture Can be used in both rats and mice by penetrating the heart. This collection thoroughly explores the dynamic and ever-developing field of hemostasis and thrombosis diagnostics and research. the ribcage. These web pages describe a method for blood collection from the saphenous vein of mice, rats, hamsters, gerbils, guinea-pigs, mink and ferrets. McGuill MW, Rowan AN. Volume Required On average, the total blood volume in the mouse is 6-8% of its body weight, or 6-8 ml of blood per 100 g of body 48 Allow clot contraction prior to centrifugation (10,000 rpm for 10 minutes at 4°C) and serum collection. Our direct replacement for the old style BD Microtainer can be customized with your preferred additive. The perfusion set up should allow collection of used aldehydebased - fixatives. B. Axillary cut down Can be used in both rats and mice. Cardiac puncture or blood collection from the posterior vena cava are good methods of terminal blood collection when an aseptic sample is needed. Volume Required. On average, the total blood volume in the mouse is 6-8% of its body weight, or 6-8 ml of blood per 100 g of body 48. 0000009150 00000 n Blood withdrawal by cardiac puncture is considered an euthanasia procedure and should be performed only after ensuring that the animal is under deep anesthesia, as evidenced by lack of response to a painful stimulus (e.g., toe or tail pinch). The size of the required sample must also be predetermined. Due to the small size of the mouse or rat, the maximum amount of blood collection must be calculated for a survival bleed. An average mouse weighing 25 grams has a total blood volume of 1.8 ml; the average rat weighing 250 grams has a total blood volume of 16 ml. 0 An anesthesized mouse is still alive and blood is free flowing with full cardiac capacity. Wet the fur with alcohol over the entire ventral area. Ocular blood collection in mice. 4.6. Spin the tubes at 5,000 rpm x 1 min. Mouse Blood Draw: Cardiac Puncture. 0000002872 00000 n Anesthetize animal. Needle size example – 22 gauge is larger than 24 gauge unless an exception has been granted, personnel working with mice at uw must complete the in-person mouse hands-on lab course. 0000003003 00000 n Veins that drain blood from other parts of the face meet in this area and form the jugular vein. blood is collected from a mouse and dried as a “spot” on special sample cards (1–4). C. The approximate blood volume of a mouse is 55-80 ml/kg of body weight (6-8% of their body weight). SOP: BLOOD COLLECTION IN THE MOUSE, INTRACARDIAC 1 I. Fully revised and updated this new edition provides the reader with agents, methods, and techniques for anesthesia and analgesia that ensure humane and successful procedural outcomes. 0000003038 00000 n This book provides a comprehensive overview of what we know about behavior, pain, and distress in laboratory animals. 2 – The mouse will be restrained in a firm scruff with your non-dominant hand, ensuring that the skin over the face is held taunt. Mouse: Immobilisation and blood sampling from the saphenous vein; i.p. Hello fellow lab rats. Table 1 – Frequently Used Sites For Blood Draws for Common Lab Animal Species. xÚb``f``Öe Ã娀ˆY8 Š In this unique volume, the authors illustrate in superb detail the anatomy of both species, permitting comparisons between them and highlighting morphological differences of clinical significance. A topical ophthalmic anesthetic should be applied prior to the procedure. 0000000736 00000 n iii. If you wait too long, cardiac puncture itself will be difficult., and you can end up with coagulated blood. Blood Collection Recommended Sites maxillary, saphenous, pedal vein, tail vein/artery, retro-orbital, jugular, cardiac puncture Injection Sites and Volumes *Use the smallest size needle gauge possible when performing injections. 27 0 obj <>stream This book provides current and detailed information which will help guide scientists, veterinarians, and students. Hyperventilation. Retro-orbital Blood Collection – Mouse SOP #PRO-006 The mouse has a large retro orbital sinus than can be used as a source of venous blood by penetrating the sinus with a glass capillary tube. The new edition of this successful reference offers both cutting-edge and classic pharmacological methods. d. Fur swabs of each sentinel mouse and fresh fecal sample from each mouse in each sentinel cage in the room are pooled. Biological effects of blood loss: Implications for sampling volumes and techniques. Bleeding procedures that should be performed on the anesthetized animal include collection from the mouse axillary plexus, cardiac puncture of the mouse, rat, hamster, or rabbit, and collection from the hamster abdominal aorta or vena cava. Blood Collection. If 30-40% of total blood volume is removed, death will result in at least 50% of animals. collection after killing Direct puncture of bladder after killing and open of the body Methods of blood collection: • Cardiac puncture • Decapitation (for steroids only) 3.3. Another common method is cardiac puncture. Blood should be withdrawn slowly.The cardiac route for blood collection is a terminal procedure. Cardiac puncture; a standard effective method requires anesthesia and is a terminal procedure while facial vein technique allows multiple collections. 6 0 obj <> endobj period of days or weeks. rÈ=-¹¤içeS™ªIâ‚qÕ¥eë.Œ›ï4n@6Þt××HqßoJXÔòrnyˆ×7€Ûí¥²5oônËÖh¡½Ô{ÖN¢ èíFÇ%u”‡m¼ÒöƒØ>j“ÏwÊã…9×6õpïÆ]¬Ò…R¼øÊ]&¬U&IÜi:ßZ¡ï. This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. startxref Found inside – Page 269There have been few studies on the effects of blood sampling on the welfare of mice and, hence, ... The white blood cell counts (WBC) in samples obtained by cardiac puncture were significantly lower than for the other three sites, ... for blood sampling in the mouse, and recommended puncture of the tail vein in the rat. 0000012904 00000 n Procedure Harvest cells from tissue, preparing a single cell suspension. Here, the basic approaches for analyzing hematopoiesis in … Often, studies require a nonsurvival, large blood sample that is collected through exsanguination via an intracardiac bleed or the caudal vena cava. Maximum blood volumes should be taken only from healthy animals (1). Version: 1.0 . This handbook will be invaluable to administrators, researchers, and employees in any animal research facility. It will also be of interest to personnel in zoos, animal shelters, and veterinary facilities. 1. This is equivalent to 40 µl/g or approximately 1 ml for an average 25 gram mouse. Thromboelastography (TEG) is a global hemostasis test, provides a dynamic real-time picture of coagulation. Generally, blood sampled from a central site, for example, cardiac, yields lower numbers of RBCs, WBCs, and platelets compared with blood sampled peripherally, consistent with previous reports 9, 13. Cardiac Puncture (Terminal Only) Required . This volume covers the field of mouse breeding and genetics at a level of detail sufficient for pursuing independent research. Anesthesia is required if blood collection is being performed either via the retro-orbital sinus or by cardiac puncture due to the distress and pain which can be caused and for … For example, for a mouse weighing 25 grams, the maximum allowable blood collection may not exceed 0.25 grams or 0.25 ml. the chest cavity, 1 mL of blood can be obtained by cardiac puncture using a 21-G needle and a syringe containing an anticoagulant. Found inside – Page 7Note the tube holder and collecting tube below the tail. Cardiac Puncture Cardiac puncture is a clean and simple way to get as much blood as possible from a mouse at the time of necropsy. It must be performed immediately after the mouse ... 6 22 This technique can be used for collecting large volumes of blood. Title: Intracardiac Puncture for Terminal Blood Collection in Mice and Rats 1.2.6 Stop needle advance when blood is visualized in syringe tip (Image 4) 1.2.7 Hold needle and syringe steady until all blood is collected 1.2.8 Perform a bilateral thoracotomy with scissor or a scalpel to insure death 2. The maximum amount of blood that can be drawn from a rodent in any 2-week period may not exceed 1% of the animal's body weight. The heart is located on the left side of the chest cavity. Please read the general principles of blood sampling page before attempting any blood sampling procedure. 3. With volumes from 10uL to 1.3mL, SAI offers a variety of collection tubes perfect for rodent blood sampling. blood collection may result in hypovolemic shock, physiological stress and even death. Thromboelastography (TEG) is a global hemostasis test, provides a dynamic real-time picture of coagulation. Mouse Facial Vein Blood Collection 1 – The appropriate size lancet will be selected (3mm for small amount of blood, 4mm for mice < 2 months, 5mm for mice 2-6 months and 5.5mm for mice > 6 months). Blood sample collection through cardiac puncture in rat Blood WithdrawalFor ppt Intracardiac.avi 33 34. Once the appropriate volume of blood is collected, release the rat and return it to the supine position in order to … Animal must be euthanized immediately after blood collection. • To ensure blood flow is not compromised, remove and perform cervical ASAP. Found inside – Page 410tached to polyethylene tubing through the midventral thorax into the heart. However, anesthesia should be administered prior to cardiac puncture. The anesthetic of choice for collection of blood for hematological examination is ether ... This method is intended for survival blood collection. Alternatively, systemic Analysis was performed on 20-μl samples of heparinized blood in the first hour following cardiac blood puncture. Retro-orbital Sinus Blood Collection The retro-orbital sinus is the site located behind the eye at the medial or lateral canthus. Rat and mouse venipuncture of the lateral tail vein iii. As with any procedure, training is critically important. Cardiac Puncture: Using a 23 g needle and 3 ml syringe, approach the The jugular vein is also an acceptable blood collection site/method but requires anesthesia. ILAR Journal; 31(4):5-20. Please refer to Table 1. This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. PART II . From an ethical perspective, this ... retro-orbital (RO) bleeds and cardiac puncture, which can add to data variability, whereas serial sampling uses just one site of collection, typically the tail or saphenous vein. Cardiac puncture for blood collection requires general anesthesia, as does collection of blood from the guinea pig anterior vena cava. This includes the hood, chamber, and plug surface. Methods of blood collection in the mouse. Blood samples are taken from the heart, preferably the ventricle, which can be accessed either via the left side of the chest, through the diaphragm, from the top of the sternum or by performing a thoracotomy. Abdominal/thoracic blood vessel. 1. The only book of its kind with in-depth coverage of the most common exotic species presented in practice, this comprehensive guide prepares you to treat invertebrates, fish, amphibians and reptiles, birds, marsupials, North American ... The improved method involved the use of a restraining board instead of manual holding. This book contains 11 chapters of significant and updated materials on what we know and what we lack and need in better understanding of Trypanosoma cruzi - a parasite that never dies - and the consequences of Chagas disease as one of the ... Cardiac blood collection (Terminal bleed) SCOPE Cardiac blood collection is used as a terminal procedure to collect a maximum volume of blood directly from the heart which is used for laboratory tests. To assist blood flow, position the mouse with the head lower than the heart. ٟw}? (Surgical plane of anesthesia is required!) c. immediately invert tube several factors, cardiac puncture mouse protocol, or cecal length. Effective 11/20/2015, Revised: 11/19/15 JC . The presence of a plexus rather than sinus in the rat can lead to greater orbital tissue damage than in the mouse. Cardiac puncture Up to 1 ml of blood can be obtained from the heart of a deeply anesthetized mouse in a terminal procedure. If > 40% of total blood volume is … Expanding on the National Research Council's Guide for the Care and Use of Laboratory Animals, this book deals specifically with mammals in neuroscience and behavioral research laboratories. %%EOF Thromboelastography (TEG) is a global hemostasis test, provides a dynamic real-time picture of coagulation. Rabbit venipuncture of the marginal ear vein iv. : Blood collection is critical for mouse research studies particularly in hemostatic testing. 4.5. Found inside – Page 89Adult mouse blood volumes and suggested sample size Volume (mL) Total blood 1.6–3.2 Single sample 0.2–0.3 Exsanguination 1–1.5 Source: Adapted from Harkness et al. ... Larger blood samples can be collected from a cardiac puncture. Brucellosis, also known as undulant fever, Mediterranean fever, or Malta fever, is an important human disease in many parts of the world. The Mandibular Vein is located under the jawbone of the mouse (as shown in the first picture). Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven ... ​The objective of this book is to concisely present information with respect to appropriate use of experimental rodents in research. Blood Collection Guidelines . ">íWeŠéŒŸÄIšffµ6§Ü]öSå So far, very little is known on how the blood collection method and the anticoagulant used influence pre-activation of mouse platelets and coagulation. Ideally, blood sampling should be minimally invasive and have minimal impact on both the wellbeing of the animals – and the experimental data , .. Retro-bulbar sinus puncture (RSP), often also referred to as retro-orbital sinus puncture , , in laboratory rodents is a commonly used technique for blood sampling. Cardiac puncture has been widely used for collection of large blood samples and variations of the technique have been described by Cubitt and Barrett (1978), Frankenberg (1979) and Waynforth (1969). difficult to locate in pigmented strains. History, Wild Mice, and Genetics, the first volume in the four volume set, The Mouse in Biomedical Research, provides information about the history, biology and genomics of the laboratory mouse (Mus musculus), as well as basic information ... Part of ACLAM's series of laboratory animal books, this text offers concise but complete coverage on rabbits and the most common rodent species, with an emphasis on biology, clinical procedures, clinical signs, and diseases and conditions. trailer Cardiac puncture is a suitable technique to obtain a single, large, good quality sample from a euthanised rat or a rat under deep terminal anaesthesia if coagulation parameters, a separate arterial or venous sample or cardiac histology are not required. Exposure of blood vessels for catheter implantation. blood collection capillary tube. 0000005515 00000 n not come in contact with the mouse’s skin or fur. Blood Collection methods in Mice and Rats (anesthetized and terminal): Cardiac Puncture: Obtainable Volume: medium – large. 0000013340 00000 n Allow to clot and keep at room temperature. A method is described for blood collection from the lateral saphenous vein. Each mouse per sentinel cage has blood collected by cardiac puncture or submental collection methods onto an Idexx opti-spot card. Introduction: The following are “good practice” guidelines recommended for blood collection volumes collection sites and needle gauges. As a model organism, the mouse recapitulates much of human hematopoiesis; however, there are some important differences. 2. This procedure requires anesthesia, which may alter parameters of the experiment. I have a quite dismal success rate with the cardiac puncture technique and I wonder if anyone here can provide some tips … For optimal health, blood draws should be limited to the lower end of the range. Responsability for the care of experimental animals. Laboratory animal facilities. The environament. Farm animal cafilities and environment. Laboratory animal care. Special practices. Health and safety responsabilities. Collect the blood in the desired collection vessel. 0000013627 00000 n Found inside – Page 444BLOOD COLLECTION AND PREPARATION OF BLOOD CELLS FROM MICE Flow cytometry analysis of murine myeloid cells was performed on blood ... Blood can be collected either by cardiac puncture or from the retro-orbital sinus, as here described. 1989. Cardiac puncture through the anterior thoracic aperture, which was originally reported by Frankeberg, was improved. This remains the Foundation Manual for information across the range of exotic pets, from small mammals, through birds, reptiles and amphibians, to invertebrates. Commoner pets, such as rabbits, rodents and budgies retain their place. The laboratory mouse is the model most frequently used in hematologic studies and assessment of blood parameters across a broad range of disciplines. Often, analysis of blood occurs in a nonterminal manner. However, the small body size of the mouse limits collection based on volume, frequency, and accessible sites. c. Cardiac Balloon™ for demonstration, teaching and practice of cardiac-puncture blood collection in rodents d. CD Mouse™ for demonstration, teaching, practice and validation of competency of cervical dislocation euthanasia in rodents e. Pink E. D. Cap™ for demonstration, teaching , practice and The saphenous vein of the thigh is visible, and one can puncture it with a 23- to 25-gauge needle, then collect the blood with a microvette capillary collection tube. This is a non-survival (terminal) procedure. Technique. 0000008451 00000 n Bacterial blood cultures were extracted for the 20 % group 24 h after CLP. In all cases, cardiac puncture may be used to obtain a single, large volume of blood from heavily anesthetized (terminal procedure only) or euthanized animals.

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