geobacter protein nanowires

B 123, 5035–5057. Biotechnol. Toward peptide-based bioelectronics: reductionist design of conductive pili mimetics. EABs transfer electrons extracellularly via conductive proteins, biofilm matrix, or nano-pili [166][167]. 114900. doi: 10.1101/114900. For example, a strain in which the gene for OmcS was deleted was incapable of Fe(III) oxide reduction (Mehta et al., 2005). Current flows between the tip and the HOPG through the pilus. Live wires: direct extracellular electron exchange for bioenergy and the bioremediation of energy-related contamination. We developed a simple strategy for functionalizing e-PNs with surface-exposed peptides. In a similar manner, G. uraniireducens produces low current densities on anodes (Rotaru et al., 2015), further indicating e-pili rather than cytochrome filaments are essential for long-range electron transport. Appl. The archaellum: an update on the unique archaeal motility structure. Microbial communities associated with anaer, direct interspecies electron transfer to Methan, between capacity for current production and syn, of micrometer scale electron transfer in heme free, Shi, L., Dong, H., Reguera, G., Beyenal, H., Lu, A., Liu, J., et, Extracellular electron transfer mechanisms between microorganisms a. Microbial nanowires: an electrifying tale. J. Phys. mBio 9, e01273–18. doi: 10.1128/JB.00331-20. Nat. More recently, it has been discovered that the G. sulfurreducens multi heme outer surface c-type cytochrome OmcS can assemble into filaments (Filman et al., 2019; Wang et al., 2019). Unable to load your collection due to an error, Unable to load your delegates due to an error, Heterologous expression of PilA pilin monomer genes in. Electroactive bacteria produce or consume electrical current by moving electrons to and from extracellular acceptors and donors. The highly intricate structures of biological systems make the precise probing of biological behaviors at the cellular‐level particularly difficult. In addition, the added pressure on the environment due to the emission of greenhouse gases has disturbed the ecological balance. a diversity of extracellular electron transfer mechanism, scholarworks.umass.edu/open_access_dissertations/129, constraints on direct interspecies electron tran, range extracellular electron transport in, conductive pili of the metal-reducing bacterium, (2019). The resulting abiotic/biotic platforms provide the basis of a range of technologies, including energy conversion and diagnostic assays. BMC Genomics 11:40. doi: 10.1186/1471-2164-11-40, Butler, J. E., Young, N. D., Aklujakr, M., and Lovley, D. R. (2012). This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. From the earliest studies (Gorby and Lovley, 1991; Lovley et al., 1993), to the present, investigations on the mechanisms for extracellular electron exchange in Geobacter species have focused primarily on cytochromes (see Lovley et al., 2004, 2011; Lovley, 2006; Shi et al., 2016, for a chronological review). Side by side comparison of the transmission electron microscope images of the filaments emanating from cells from strain CL-1 and wild-type cells (Leang et al., 2013) demonstrate that some of the filaments associated with the CL-1 strain have a greater diameter and different morphology than the filaments associated with wild-type G. sulfurreducens (Figure 9). A new type of energy-harvesting device, based on protein nanowires from the microbe Geobacter sulforreducens, can generate a sustained power output by producing a moisture gradient across the . These studies suggest broad possibilities for tailoring e-PN properties for diverse applications. Walker1,2 3 1Department of Microbiology, University of Massachusetts, Amherst, MA 01003 4 2Institute for Applied Life Sciences, University of Massachusetts, Amherst, MA 01003 5 *dlovley@microbio.umass.edu 6 7 ABSTRACT 8 The study of electrically conductive protein nanowires in Geobacter sulfurreducens has Next, several cellular‐level nanowire‐enabled applications in biophysical dynamics probing, physiological or biochemical sensing, and biological activity modulation are highlighted. doi: 10.1128/JB.06366-11. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. A new, highly sensitive chemical sensor uses protein nanowires. Environ. Technol. The design of new protein nanowires based on the known M. hungatei archaellum structure could be a major advance over the current empirical design of synthetic protein nanowires from electrically conductive bacterial pili. doi: 10.1128/mBio.00159-11, Nevin, K. P., and Lovley, D. R. (2000). | Multiple lines of evidence suggest that OmcS is not important in long-range electron transport. doi: 10.1159/000115849, Klimes, A., Franks, A. E., Glaven, R. H., Tran, H. T., Barrett, C. L., Qiu, Y., et al. Images in (D) reproduced with permission from (Adhikari et al., 2016). Environ. In contrast, G. uraniireducens uses an electron shuttle for long-range electron transport and has a pilin with low aromatic amino acid abundance (Tan et al., 2016b). laments examined were 3nm in diameter, as expected for e-pili, expressing different PilA pilin monomer genes in, reagents highly specic for the His-tag (, on the carboxyl end resulted in a strain that exp, suggesting a lack of OmcS laments. Both e-pili and OmcS filaments offer design options for the synthesis of protein-based ‘green’ electronics, which may be the primary driving force for the study of these structures in the near future. (2019). Deletion of omcS had no significant impact on current production in such systems (Nevin et al., 2009; Malvankar et al., 2011, 2012). Adv. 66, 2248–2251. Phys. 50 kDa) and PilA (at ca. The editor and reviewers' affiliations are the latest provided on their Loop research profiles and may not reflect their situation at the time of review. doi: 10.1016/j.bbabio.2011.01.003, Reguera, G., McCarthy, K. D., Mehta, T., Nicoll, J. S., Tuominen, M. T., and Lovley, D. R. (2005). Strain KN400 expresses much less OmcS that the, ). No filaments were observed when the gene for the PilA pilin monomer was deleted from the wild-type strain (Reguera et al., 2005). Geobacter species also are effective in the bioremediation of organic (Lovley et al., 1989; Anderson et al., 1998; Rooney-Varga et al., 1999) and metal (Lovley et al., 1991; Lovley, 1995, 2003; Anderson et al., 2003) contaminants in subsurface environments. 52, 10241–10253. (2006). Chem. Individual filaments emanating from cells that were also not labeled were observed in both wild-type cells (Figure 7) and a strain in which omcS was deleted (Leang et al., 2010). 17, 648–655. When a strain of G. sulfurreducens was constructed in which one of the tyrosines in PilA thought to be important in electron transport was replaced with an alanine the conductivity of the individual filaments decreased 5-fold. Electrochemically induced formation of cytochrome c oligomers at soft interfaces. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. This volume also explores the toxicity of nanomaterials in microbes and their effect on human health and the environment. Denaturing gradient gel electrophoresis of 16S rDNA sequences amplified with bacterial or Geobacteraceae -specific primers indicated significant differences in the composition of the microbial communities at the different sites. . PMC Provided by: University of Massachusetts Amherst. However, the height of these filaments was 2–3 nm, too thin for OmcS. Images reproduced with permission from (Leang et al., 2013). In addition this book also offers a distinctive understanding of nanobiotechnology from researchers and educators and gives a comprehensive facility for future developments and current applications of nanobiotechnology. Abundant literature demonstrates that G. sulfurreducens expresses e-pili, which are required for long-range electron transport to Fe (III) oxides and through conductive biofilms. Syntrophic growth with direct interspecies electron transfer between pili-free Geobacter species. The size of the particles (5.6 nm) is highly reproducible and comparable with the size of a single protein. At the time, these observations were made the possibility of OmcS filaments was under serious consideration (Tran, 2009), but the discontinuous immunogold labeling for OmcS along filaments was interpreted as association of OmcS with e-pili (Leang et al., 2010), an interpretation consistent with other results presented below. By using highly sophisticated energy dispersive X-ray spectroscopy (EDXS) combined with high angle annular dark field (HAADF) transmission electron microscopy enabling detection, identification, and localization of chemical compounds on the surface at nearly atomic spatial resolution, we analyze Fe spatial distribution within Geobacter sulfurreducens community. Gutermann, T., and Gazit, E. (2018). Appl. The concept of long-range electron transport through microbially produced protein filaments with nanometer diameters (Reguera et al., 2005) has provided new insights into microbial physiology and ecology (Lovley, 2017b,c,d). Many of the extracellular electron tra, ). Microbiol. (2018). (2018). The Department of Energy's Office of Science is the single largest supporter of basic research in the physical sciences in the United States and is working to address . Careers. 103, 1535–1544. Before coming to UMass Amherst, Yao had worked for years at Harvard University, where he engineered electronic devices with silicon nanowires. Decorating the outer surface of microbially produced protein nanowires with peptides. The pili in the strains with heterologously expressed pilin monomer genes have a diameter that is the same as wild-type pili (3 nm) and inconsistent with the diameter of OmcS filaments (4 nm). Rep. 3, 211–217. Oligomerization of cytochro, (2010). Environ. Keywords: Although it is well established that a periplasmic c-type cytochrome (c-Cyt) network plays a critical role in regulating EET efficiency, the understanding of the network in terms of structure and electron transfer activity is obscure and partial. Sci. The potential applications of electrically conductive protein nanowires (e-PNs) harvested from Geobacter sulfurreducens might be greatly expanded if the outer surface of the wires could be modified to confer novel sensing capabilities or to enhance binding to other materials. Biol. Environ. Figure 2. Conductive nanowires have also been confirmed in the oxygenic cyanobacterium Synechocystis PCC6803 and a thermophilic, methanogenic coculture consisting of Pelotomaculum thermopropionicum . doi: 10.1128/AEM.02954-12, Smith, J. "The Geobacter cells are making these protein fibers naturally to breathe certain metals. These results suggest that Geobacter spp. e outer surface localization of OmcS, cou, of a synthetic pilin monomer with the same, Early (circa 2002) transmission electron micr. Conducting p, the pili with peptide tags over that of the wild-type.

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